WebNov 25, 2024 · As mentioned above, we confirmed the autophagy activation signaling by measuring pAMPK (T172), pULK1 (S757), pULK1(S555), pS6 (S235/236) and Atg5-12 conjugation. When mice were treated with HCQ to block autophagy flux, besides p62 and LC3II accumulation, we also observed that Atg5-12 conjugated form was increased in the … WebMar 15, 2024 · Representative western blotting for autophagy-related genes (LC3II, P62, ATG7, Beclin-1, pmTORC1 S2448, pULK1 S757 and pAMPK T172) and the reference protein, GAPDH is shown (A,C,E).
Phospho-ULK1 (Ser757) Antibody Cell Signaling Technology
WebEtOH altered ULK1 S555 and S757 phosphorylation in a time- and AMPK-dependent manner. The activation of AMPK and ULK1 was associated with increased BECN1 (S93, … WebJul 1, 2024 · Protein levels of pULK1 S757 in retinas from cattle inoculated with BSE-H and BSE-L were comparable to basal levels detected in retinas from sham-inoculated cattle … is there a robot vacuum for stairs
Disruption of the polyubiquitin gene - ScienceDirect
WebFeb 13, 2024 · Primary antibodies against Met, pMet (Y1234/1235), p62, LC3, mTOR, pmTOR (S2448), pULK1 (S757), Atg5, Beclin-1, NBR1, and secondary horseradish peroxidase-conjugated goat anti-rabbit and anti ... WebDec 6, 2024 · Reactivation of mTORC1 in full medium within 1 h was observed by phosphorylation of downstream substrates pULK1 (S757), pWIPI2 (S413), and pAMBRA1 (S52), ... Interestingly, although we observed reduced ULK1 S757 phosphorylation in USP32 KO cells and also accumulation of nuclear TFEB as a consequence of reduced mTORC1 … WebJul 15, 2016 · First, Lcn2 reduced pULK1 S555, increased pULK1 S757 and reduced LC3-II levels determined by Western blotting. We validated the use of DQ-BSA to assess autolysosomal protein degradation and this together with MagicRed cathepsin B assay indicated that Lcn2 reduced lysosomal degradative activity. iis wordpress htaccess